Determination of erythrocyte Glutathione S-Transferase activity in individuals with gastric and colon cancer

Glutathione-S-Transferase (GST) in human cells has great importance the detoxification mechanism of carcinogenic chemicals. Therefore, may be a useful tumor marker. This study examines whether GST activity Gastric cancer (GC) and Colon Cancer (CC) is helpful marker diagnosing monitoring disease course. was investigated patients with CC GC healthy individuals. Erythrocyte isolation performed 3 ml blood samples from volunteers aged 18-75 years. Hemoglobin amounts were determined standard graph drawn by conversion methemoglobin to cyanmethemoglobin presence cyanide at 540 nm. Glutathione S-transferase measuring amount enzyme that catalyzes 1 µmol S-(2,4-dinitrophenyl) glutathione formed per minute using 1-Chloro-2,4-Dinitrobenzene. The mean values activities GC, respectively; (1.28 ± 0.23 U/gHb; 1.20 0.30 U/gHb), significantly higher when compared individuals (0.59 0.13 U/gHb) (p < 0.05). colon cancer, measured as (1.56 after chemotherapy, than before (1.09 0.12 (1.53± 0.24 chemotherapy gastric value (0.97± Our results show change can used biomarker monitor course response chemotherapy.